What Must Be Registered - Institutional Biosafety Committee Skip Navigation

What Must Be Registered

Complete guidelines regarding what must be registered with the Institutional Biosafety Committee.
Experiments involving the following must be registered with the Institutional Biosafety Committee (IBC):
  • Pathogens affecting humans, animals or plants;
  • Materials potentially containing human pathogens (for example, unfixed human specimens, human blood)
  • Recombinant DNA molecules including virus vectors (recombinant DNA research registration checklist)
  • Human cell lines that are not well-characterized or require Risk Group 2 containment
  • Generation of de novo transgenic animals: defined as the addition of foreign DNA or subtraction of a portion of the animal genome using recombinant DNA technology. The breeding of transgenic animals to generate additional transgenic offspring does not require IBC approval. Those transgenic animals that already exist or which have been purchased also do not require IBC approval.
  • All research involving the use of recombinant or synthetic nucleic acid molecules containing no more than two-thirds of the genome of any eukaryotic virus, or biohazards.
  • Animal Subjects: All research involving the use of recombinant molecules or biohazards in whole animals requires both IBC and IACUC approval.
  • Human Subjects: Any research involving the introduction of recombinant molecules or biohazards into human subjects must be approved by the IBC and by the IRB.

Human Cells and Tissues

Human and non-human primate cells should be handled using Risk Group 2 (RG-2) practices and containment. All work should be performed in a biosafety cabinet and all material should be decontaminated by autoclaving or disinfection before discarding. Appropriate training in the handling of Blood-Borne Pathogens and up-to-date hepatitis B vaccinations may be required.

Select Agents and Toxins

Select agents are specific pathogens and toxins that have the potential to pose a severe threat to public health and safety as defined by the USA PATRIOT Act and the Public Health Security and Bioterrorism Preparedness and Response Act of 2002. The institution must be registered with the CDC and/or USDA before these materials are obtained, used or stored.

Risk Group One (RG-1)

Risk Group One (RG-1) agents are usually not placed on a list but include all microorganisms that do not pose a health risk to healthy adult humans. It must not be assumed that an organism not listed as a RG 2, 3, or 4 agent is an RG-1 agent; emerging or unknown organisms should be treated as biohazardous until research proves otherwise. Examples of agents in RG-1 are: Bacillus subtilus, infectious canine hepatitis viruses; influenza reference strains A/PR/8/34, A/WS/33, Escherichia coli K12, Saccharomyces cerevisiae, and other agents listen in Appendix C-II of the NIH Guidelines for Research Involving Recombinant DNA Molecules; and other vectors such as Baculovirus.

Additional low-risk oncogenic viruses identified as RG-1 agents
  • Adenvirus 7-Simian virus 40 (Ad7-SV40)
  • Avian Leucosis virus
  • Bovine leukemia virus
  • Bovine papilloma virus
  • Chick-embryo-lethan orphan (CELO) virus
  • Dog sarcoma virus
  • Guinea pig herpes virus
  • Lucke (frog) virus
  • Hamster leukemia virus
  • Marek’s disease virus
  • Mason-Pfizer monkey virus
  • Mouse mammary tumor virus
  • Murine leukemia virus
  • Murine sarcoma virus
  • Polyoma virus
  • Rat leukemia virus
  • Rous sarcoma virus
  • Shope vibroma virus
  • Shope papilloma virus
  • Simian virus 40 (SV-40)

Risk Group Two (RG-2)

RG-2 Inspection Form
RG-2 agents are of moderate potential hazard to healthy adult humans and the environment. Such agents may produce disease of varying degrees of severity from accidental inoculation, injection, or other means of cutaneous penetration but can usually be adequately and safely contained by ordinary laboratory techniques. Some agents may cause disease by contact or respiratory routes, but they are self-limiting and do not cause a serious illness, such as the cause of the common cold, the rhinoviruses. The following organisms have been identified as RG-2 agents:
Bacteria
  • Acinetobacter baumannii
  • Actinobacillus spp
  • Actinomyces pyogenes
  • Aeromonas hydrophila
  • Amycolata autotrophica
  • Archanobacterium haemolyticum
  • Bacteroides spp.
  • Borrelia recurrentis
  • Burkholderia (except those in RG-3)
  • Campylobacter coli
  • Clostridium chauvoei
  • Corynebacterium diphtheria
  • Dermatophilus congolensis
  • Edwardsiella tarda
  • Erysipelothrix rhusiopathiae
  • Escherichia coli
  • Haemophilus ducreyi
  • Helicobacter pylori
  • Klebsiella spp.
  • Leptospira interrogans
  • Listeria spp.
  • Moraxella spp.
  • Mycobacterium spp.
  • Nocardia asteroids
  • Pasteurella spp.
  • Plesiomonas shigelloides
  • Proteus spp.
  • Salmonella spp.
  • Sphaerophorus necrophorus
  • Staphylococcus aureus
  • Streptobacillus moniliformis
  • Streptococcus spp
  • Treponema pallidum
  • Vibrio cholera
  • Yersinia enterocolitica
Fungal agents
  • Blastomyces dermatitidis
  • Cladosproium bantianum
  • Cryptococcus neoformans
  • Epidermophyton spp.
  • Exophiala dermatitidis
  • Fonsecaea pedrosoi
  • Microsporum spp.
  • Ochroconis gallopavum
  • Paracoccidiodes brasilensis
  • Penicillium marneffei
  • Sporothrix schenckii
  • Trichophyton spp.
Parasitic agents
  • Ancylostoma spp.
  • Ascaris spp.
  • Babesia spp.
  • Brugia spp.
  • Coccidian spp.
  • Cryptosporidium spp.
  • Cysticercus cellulosae
  • Echinococcus spp.
  • Entamoeba histolyitca
  • Enterobius spp.
  • Fasciola spp.
  • Giardia spp.
  • Heterophyes spp.
  • Hymenolepis spp.
  • Isospora spp.
  • Leishmania spp.
  • Loa loa filarial
  • Microsporidium spp.
  • Naegleria fowleri
  • Necator spp.
  • Onchoerca spp.
  • Plasmodium spp.
  • Sarcocystis spp.
  • Schistosoma spp.
  • Strongyloides spp.
  • Taenia solium
  • Toxocara spp.
  • Trichinella spiralis
  • Trypanosoma spp.
  • Wuchereria bancrofti