Academics

Roger S. Rowlett
Professor

B.S. (Biochemistry) 1976, University of Alabama
Ph.D. (Chemistry) 1981, University of Alabama

E-mail:rrowlett@mail.colgate.edu
(315) 228-7245

Teaching Interests: Biochemistry, Analytical Chemistry, General Chemistry

Research Interests: Enzymology, the isolation, purification, and characterization of enzymes, especially experimental kinetics; Protein Engineering, the specific alteration of enzyme structure using recombinant DNA methods; X-ray Crystallography, the determination of three-dimensional protein structures from X-ray diffraction by single protein crystals.

Selected Publications:

• Sarah L. Binkley, Christopher J. Ziegler, Richard S. Herrick, and Roger S. Rowlett, “Specific Derivitization of Lysozyme in Aqueous Solution with a Rhenium(I) Tricarbonyl Fragment,” Chem. Comm. 2010, in press.
• Roger S. Rowlett, "Structure and Catalytic Mechanism of the ß-Carbonic Anhydrases," Biochim. Biophys. Acta 2010, in press.
• Roger S. Rowlett, Chingkuang Tu, Joseph Lee*, Ariel G. Herman*, Douglas A. Chapnick*, Shalini H. Shah*, and Peter C. Gareiss*, “Allosteric Site Variants of Haemophilus influenzae ß-Carbonic Anhydrase,” Biochemistry 2009, 48, 6146-6156.
• Jeff D. Cronk, Roger S. Rowlett, Kam Y. J. Zhang, Chingkuang Tu, James A. Endrizzi, Joseph Lee,* Peter C. Gareiss,* and Jeffrey R. Preiss,* "Identification of a novel non-catalytic bicarbonate binding site in eubacterial  ß-carbonic anhydrase," Biochemistry 2006, 4351-4361.
• Daniel Clark, Roger S. Rowlett, John R. Coleman, and Daniel F. Klessig, "Complementation of the yeast deletion mutant ?NCE103 by members of the ß class of carbonic anhydrases is dependent on carbonic anhydrase activity rather than on antioxidant activity," Biochem. J. 2004, 379, 609-615.
• Roger S. Rowlett, Chingkuang Tu, Paul S. Murray,* and Joy E. Chamberlin,* "Examination of the role of Gln-158 in the Mechanism of CO₂ Hydration Catalyzed by ß-Carbonic Anhydrase from Arabidopsis thaliana," Arch. Biochem. Biophys., 2004, 425, 25-32.
• Roger S. Rowlett, “Elements of a Competitive NIH-AREA proposal,” Council on Undergraduate Research Quarterly 2004, 24, 120-124.
• Chingkuang Tu, Roger S. Rowlett, Brian C. Tripp, James G. Ferry, and David N. Silverman, "Chemical Rescue of Proton Transfer in Catalysis by Carbonic Anhydrases in the ß- and ?-Class," Biochemistry 2002, 41, 15429-15435.
• Roger S. Rowlett, Chingkuang Tu, Melissa M. McKay,* Jeffrey R. Preiss,* Rebecca J. Loomis,* Katherine A. Hicks,* Robb J. Marchione,* Jacob A. Strong,* George S. Donovan, Jr.,* and Joy E. Chamberlin,* "Kinetic Characterization of Wild-Type and Proton Transfer-Impaired Variants of ß-Carbonic Anhydrase from Arabidopsis thaliana," Arch. Biochem. Biophys. 2002, 404, 197-209.
• Kwang-Hwan Jhee, Li-Hong Yang, S. Ashraf Ahmed, Peter McPhie, Roger Rowlett, and Edith Wilson Miles, "Mutation of an Active Site Residue of Tryptophan Synthase ßS377 Alters Cofactor Chemistry," J. Biol. Chem. 1998, 273, 11417-11422.
• C. Larry Bering, Jennifer S. Kuhns, and Roger S. Rowlett, "Purification of Bovine Carbonic Anhydrase by Affinity Chromatography: An Undergraduate Biochemistry Laboratory Experiment," J. Chem. Ed. 1998, 75, 1021-1024.
• Roger Rowlett, Lihong Yang, S. Ashraf Ahmed, Peter McPhie, Kwang-Hwan Jhee, and Edith W. Miles, "Mutations in the Contact Region between the a and ß Subunits of Tryptophan Synthase alter Subunit Interaction and Intersubunit Communication," Biochemistry1998, 37, 2961-2968.
• Roger S. Rowlett, Mark R. Chance, Michael D. Wirt, Dean E. Sidelinger, Jill R. Royal,* Melanie Woodroffe,* Ying-Fuh A. Wang,* Rajib P. Saha,* and Michael G. Lam*, "Kinetic and Structural Characterization of Spinach Carbonic Anhydrase," Biochemistry 1994, 33, 13967-13976.

Asterisks indicate undergraduate student co-authors who conducted their research in collaboration with a faculty member at Colgate.